Effects of Curcumin, Resveratrol and Ursodeoxycholic Acid on Ethinylestradiol and Chlorpromazine- Induced Intrahepatic Cholestasis in Rats

The present study was designed to examine the hepatoprotective effects of curcumin (CMN), resveratrol (RSV) and ursodeoxycholic acid (UDCA) against ethinylestradiol and chlorpromazine -induced intrahepatic cholestasis. Ethinylestradiol (EE) and chlorpromazine HCl (CPZ) were administered orally once daily simultaneously with the tested compounds for one week. At the end of the experimentation period, animals were sacrificed. Blood and liver samples were obtained, serum was used for the determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST), direct& indirect bilirubin, gamma-glutamyltransferase (γ-GT) and cholesterol. Liver samples were used for hiatopathological examination. The results demonstrate that CMN showed marked reduction in all biochemical indices of cholestasis while RSV failed to ameliorate EE-CPZ-induced changes. UDCA produced limited protective effects against EE-CPZinduced cholestatic injury. This study suggests that CMN has a potent anticholestatic effect in case of EE-CPZ-administration.